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Prednisone and mesalamine together has been shown to improve symptoms of MS. This combination has been used in our laboratory to improve disease activity in a mouse model of MS [46].
Although the anti-inflammatory properties of this combination are encouraging, there is some controversy as to whether the combination would prevent progression of the disease. In some studies, it has been shown that the combination of mesalamine and anti-inflammatory medications did not prevent disease progression [10, 47, order prednisone online 48]. However, the combination of mesalamine Propranolol capillary hemangioma and anti-inflammatory medication did reduce disease activity in a mouse model of PD, and we believe that the beneficial effects of mesalamine and anti-inflammatory medications could be transferred to the mouse model of MS. Therefore, we decided to investigate the efficacy of combination in mouse model MS.
METHODS
Animals and treatment
Male ICR mice (n=24) (Charles River Laboratories, Wilmington, MA, USA) were used as controls. Mice weighing 200–250 g were obtained from Charles River Laboratories (Wilmington, MA, USA). All mice Best price for tretinoin cream .1 were housed at the University of Michigan Veterinary Medical Sciences Center at an ambient temperature of 21°C with a 12 h light/dark cycle and on a 12 h light/dark light cycle.
The experimental protocol was approved by the Institutional Animal Care and Use Committee of the University Michigan. All experimental procedures were performed in accordance with the International Conference on Harmonisation Guidelines and with the NIH Guide for Care and Use of Laboratory Animals the American College of Laboratory Animal Science's Guide for the Use of Laboratory Animals.
Mesalamine and + anti-inflammatory drugs treatment
Mesalamine was obtained from Sigma-Aldrich (St. Louis, MO, USA). Mesalamine (2.5 mg/kg i.p.) or + mesalamine 5 mg/kg was administered intraperitoneally (i.p.) on day 1. days 2, 4 and 5 mesalamine + anti-inflammatory drugs were administered intraperitoneally (i.p.). The following drugs (Sigma-Aldrich) were used: aspirin, ibuprofen, erythromycin, dapsone, diclofenac, erythromycin + ibuprofen, diclofenac anti-inflammatory drugs. The doses of mesalamine and + anti-inflammatory drugs were chosen to mimic t